![]() burnetii antibodies and bulk tank milk (BTM) samples from 252 sheds have been tested by real time polymerase chain reaction and ELISA for the presence of C. ![]() Blood serum samples from abortion cases from 1010 sheds have been tested by ELISA for the presence of C. The purpose of this study was to describe prevalence and spatial distribution of Coxiella burnetii infections in dairy cow sheds in Latvia and to investigate risk factors contributing to C. The new genotypes and sequence types need further research particularly into their pathogenicity to humans. Two MST sequence types were recorded: ST16 and a novel sequence (ST61). Overall, five previously known MLVA genotypes (I, J, BG, BE, and NM) and three new ones (proposed as PL1, PL2, and P元) were identified. Among real-time PCR–positive samples, 49 specimens obtained from 49 cattle herds and 8 samples of purchased dairy products were selected for genotyping. burnetii shedding was confirmed in 31.54% of tested cattle herds as well as in 69.16% of consumer milk and dairy products. Furthermore, 30 consumer milk samples, including 10 from vending machines and 77 dairy products were also analyzed. Overall, 1,439 samples from 279 herds were tested including: 897 individual milk specimens, 101 bulk tank milk samples, 409 genital tract swabs and 32 placentas. burnetii DNA was detected using a commercial real-time PCR kit, targeting the IS1111 element. The aims of this study were to evaluate the prevalence of Coxiella (C.) burnetii shedding in Polish dairy cattle herds and to identify the pathogen’s genotypes and sequence types (STs) using multiple-locus variable number tandem repeat analysis (MLVA) and multispacer sequence typing (MST) methods. Q fever is a worldwide zoonotic disease reported in humans and many animal species including cattle.
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